Details:

Notes

For research use only.

Additional_information

MW: 35.5 kDa; Buffer composition: Tris-based buffer with 50% glycerol.

Description

Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein(ssbF) expressed in E.coli

Specifications

Uniprot ID: P18310; Host: Escherichia coli (strain K12); Expression system: E.coli; Expression region: 2-179aa; Sequence information: Full Length of Mature Protein; Tag: N-terminal 6xHis-SUMO-tagged; Purity: Greater than 90% as determined by SDS-PAGE.

Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.

Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Escherichia coli Plasmid-derived single-stranded DNA-binding protein (ssbF)

Product description: