Plasmid maxiPREP Kit

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Product description:

Price: 415.00

Size: 10 rxns

Catalog no: PDP03-0010

Details:

Elution volume

NA

Operation time

NA

Format

NA

Uses

For laboratory use

Yield

Up to 850 μg of plasmid

Storage

Store at room temperature.

Sample

Up to 200 ml bacterial cells

Shelflife

Use before expiry date given on the label

Description

Plasmid maxiPREP Kit is used for isolating the plasmid DNA from the cultured bacterial cells.

Tips

Our specialists recommend you to follow carefully the pre-registered instructions for Plasmid maxiPREP Kit

Contents

Buffer M1 Buffer M2 Buffer M3 Buffer W1 Buffer W2 *Add 100 ml of ethanol prior to initial use. Buffer E RNase A (50mg/ml) MX Column

Ordering

To order Plasmid maxiPREP Kit, please use the Cat. Nr.PDP03-0010 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information

PHYSICAL AND CHEMICAL PROPERTIES

Buffer M1 Form: liquid Miscibility with water: immiscible pH:7.6 // Buffer M2 Form: liquid Miscibility with water: immiscible pH:13 // Buffer M3 Form: liquid Miscibility with water: immiscible pH:4.5 // Buffer W1 Form: liquid Miscibility with water: immiscible pH:7.0 // Buffer W2 Form: liquid Miscibility with water: immiscible pH:7.5 // BufferE Form: liquid Miscibility with water: immiscible pH:8.5 // RNase A Form: liquid Miscibility with water: immiscible pH:6.8

Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.